Sec Form 3 Filing - Thompson Travis Alvin @ BICYCLE THERAPEUTICS plc - 2023-06-22

FORM 3 Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person Thompson Travis Alvin 2. Issuer Name and Ticker or Trading Symbol BICYCLE THERAPEUTICS plc [ BCYC] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) _____ Director _____ 10% Owner X __ Officer (give title below) _____ Other (specify below) Chief Accounting Officer (Last) (First) (Middle) C/O BICYCLE THERAPEUTICS PLC, BLOCKS A & B, PORTWAY BUILDING 3. Date of Earliest Transaction (MM/DD/YY) 06/22/2023 (Street) CAMBRIDGE, X0CB21 6GS 4. If Amendment, Date Original Filed (MM/DD/YY) 6. Individual or Joint/Group Filing (Check Applicable Line) __ X __ Form filed by One Reporting Person _____ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price Ordinary Shares 8,094 ( 1 ) D

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares Employee Stock Option (right to buy) $ 1.83 ( 3 ) ( 2 ) 09/17/2028 Ordinary Shares 3,929 D Employee Stock Option (right to buy) $ 8.03 ( 3 ) ( 2 ) 04/24/2029 Ordinary Shares 456 D Employee Stock Option (right to buy) $ 9.82 ( 4 ) 01/02/2030 Ordinary Shares 14,063 D Employee Stock Option (right to buy) $ 17.95 ( 5 ) 01/04/2031 Ordinary Shares 23,500 D Employee Stock Option (right to buy) $ 60.87 ( 6 ) 01/03/2032 Ordinary Shares 9,000 D Employee Stock Option (right to buy) $ 29.6 ( 7 ) 01/03/2033 Ordinary Shares 40,000 D

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other Thompson Travis Alvin C/O BICYCLE THERAPEUTICS PLC BLOCKS A & B, PORTWAY BUILDING CAMBRIDGE, X0CB21 6GS Chief Accounting Officer Signatures /s/ Jason Minio, Attorney-in-Fact 07/03/2023 Signature of Reporting Person Date

Explanation of Responses:

( 1 )Represents restricted stock unit ("RSU") awards. 3,094 RSUs shall vest in eleven equal quarterly installments commencing on July 3, 2023. 5,000 RSUs shall vest one-fourth (1/4) of the total number of RSUs on January 3, 2024, and the remaining RSUs shall vest in 12 equal quarterly installments thereafter. ( 2 )Fully vested and exercisable. ( 3 )This exercise price is in US Dollars and reflects the conversion of GBP to USD at an exchange rate of $1.2605 to GBP 1.00 as of May 22, 2019. ( 4 )This option vested one-fourth (1/4) of the total number of shares on January 2, 2021, and the remaining shares vested or shall vest in 36 equal monthly installments at the end of each calendar month thereafter. ( 5 )This option vested one-fourth (1/4) of the total number of shares on January 4, 2022, and the remaining shares vested or shall vest in 36 equal monthly installments thereafter. ( 6 )This option vested one-fourth (1/4) of the total number of shares on January 3, 2023, and the remaining shares vested or shall vest in 36 equal monthly installments thereafter. ( 7 )This option shall vest one-fourth (1/4) of the total number of shares underlying the option on January 3, 2024, and the remaining shares vest in 36 equal monthly installments thereafter.

Remarks: Exhibit List - Exhibit 24 - Power of Attorney

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

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