Sec Form 4 Filing - Cui Xiangmin @ ALPINE IMMUNE SCIENCES, INC. - 2024-05-18

FORM 4 Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person Cui Xiangmin 2. Issuer Name and Ticker or Trading Symbol ALPINE IMMUNE SCIENCES, INC. [ ALPN] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) X __ Director X __ 10% Owner _____ Officer (give title below) _____ Other (specify below) (Last) (First) (Middle) C/O ALPINE IMMUNE SCIENCES, INC., 188 EAST BLAINE STREET, SUITE 200 3. Date of Earliest Transaction (MM/DD/YY) 05/18/2024 (Street) SEATTLE, WA98102 4. If Amendment, Date Original Filed (MM/DD/YY) 6. Individual or Joint/Group Filing (Check Applicable Line) _____ Form filed by One Reporting Person __ X __ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price Common Stock 05/18/2024 U 6,582,380 D 0 I See Footnote ( 3 ) Common Stock 05/18/2024 U 1,735,544 D 0 I See Footnote ( 4 )

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares Stock Option (Right to buy) $ 5.7008 ( 5 ) 05/20/2024 D 7,650 ( 6 ) 01/17/2029 Common Stock 7,650 ( 7 ) ( 8 ) 0 D Stock Option (Right to buy) $ 3.6233 ( 5 ) 05/20/2024 D 7,650 ( 6 ) 01/01/2030 Common Stock 7,650 ( 7 ) ( 8 ) 0 D Stock Option (Right to buy) $ 13.45 ( 5 ) 05/20/2024 D 7,650 ( 6 ) 01/03/2031 Common Stock 7,650 ( 7 ) ( 8 ) 0 D Stock Option (Right to buy) $ 13.89 ( 5 ) 05/20/2024 D 10,000 ( 6 ) 01/02/2032 Common Stock 10,000 ( 7 ) ( 8 ) 0 D Stock Option (Right to buy) $ 7.43 ( 5 ) 05/20/2024 D 10,000 ( 6 ) 01/02/2033 Common Stock 10,000 ( 7 ) ( 8 ) 0 D Stock Option (Right to buy) $ 19 ( 5 ) 05/20/2024 D 17,500 ( 9 ) 01/01/2034 Common Stock 17,500 ( 7 ) ( 8 ) 0 D

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other Cui Xiangmin C/O ALPINE IMMUNE SCIENCES, INC. 188 EAST BLAINE STREET, SUITE 200 SEATTLE, WA98102 X X Decheng Capital China Life Sciences USD Fund III, L.P. UGLAND HOUSE, PO BOX 309 GRAND CAYMAN, E9KY1-1104 X Decheng Capital Management III (Cayman), LLC UGLAND HOUSE, PO BOX 309 GRAND CAYMAN, E9KY1-1104 X Decheng Capital Global Healthcare Fund (Master), LP UGLAND HOUSE, PO BOX 309 GRAND CAYMAN, E9KY1-1104 X Decheng Capital Global Healthcare GP, LLC UGLAND HOUSE, PO BOX 309 GRAND CAYMAN, E9KY1-1104 X Signatures Decheng Capital China Life Sciences USD Fund III, L.P., By Decheng Capital Management III (Cayman), LLC, its General Partner, By /s/ Xiangmin Cui, Manager 05/20/2024 Signature of Reporting Person Date Decheng Capital Management III (Cayman), LLC, By /s/ Xiangmin Cui, Manager 05/20/2024 Signature of Reporting Person Date Decheng Capital Global Healthcare Fund (Master), LP, By Decheng Capital Global Healthcare GP, LLC, its General Partner, By Decheng Capital SV LLC, its Manager, By Decheng Capital LLC, its Manager, By /s/ Xiangmin Cui, Manager 05/20/2024 Signature of Reporting Person Date Decheng Capital Global Healthcare GP, LLC, By Decheng Capital SV LLC, its Manager, By Decheng Capital LLC, its Manager, By /s/ Xiangmin Cui, Manager 05/20/2024 Signature of Reporting Person Date /s/ Xiangmin Cui 05/20/2024 Signature of Reporting Person Date

Explanation of Responses:

( 1 )This Form 4 reports securities disposed of pursuant to the Agreement and Plan of Merger (the Merger Agreement), dated April 10, 2024, among Alpine Immune Sciences, Inc., a Delaware corporation (the Company), Vertex Pharmaceuticals Incorporated, a Massachusetts corporation (Parent), and Adams Merger Sub, Inc., a Delaware corporation and a wholly owned subsidiary of the Parent (Merger Sub). Pursuant to the Merger Agreement, Merger Sub completed a tender offer for share s of the Companys Common Stock, par value $0.001 per share (Company Common Stock), effective May 18, 2024 (the Offer Closing), and thereafter merged with and into the Company (the Merger, and together with the Offer Closing, the Transactions), effective May 20, 2024 (the Effective Time), with the Company surviving the Merger as a wholly owned subsidiary of Parent. ( 2 )(Continued from Footnote 1) In connection with the consummation of the Transactions, each share of the Company Common Stock was either (x) purchased at the Offer Closing for $65.00 in cash per share (the Offer Price), without interest thereon and subject to any applicable tax withholding or (y) automatically converted in the Merger at the Effective Time into the right to receive the Offer Price in cash, without interest thereon (the Merger Consideration), subject to any applicable tax withholding. ( 3 )These securities are owned directly by Decheng Capital China Life Sciences USD Fund III, L.P. ("Fund III"). Xiangmin Cui is the manager of Decheng Capital Management III (Cayman), LLC, the general partner of Fund III. Dr. Cui disclaims beneficial ownership of these securities, except to the extent of his pecuniary interest therein. ( 4 )These securities are owned directly by Decheng Capital Global Healthcare Fund (Master), LP ("Healthcare"). Xiangmin Cui is the indirect managing member and ultimate beneficial owner of Decheng Capital Global Healthcare GP, LLC, the general partner of Healthcare. Dr. Cui disclaims beneficial ownership of these securities, except to the extent of his pecuniary interest therein. ( 5 )The exercise price is equal to the closing price per share of Common Stock as reported on the Nasdaq Global Market on the date of grant. ( 6 )The option to purchase shares of Company Common Stock (the Company Option) is fully vested and exercisable. ( 7 )Pursuant to the Merger Agreement, as of immediately prior to the Effective Time, each option to purchase shares of Company Common Stock (a Company Option) that was then outstanding but not then vested or exercisable became immediately vested and exercisable in full. At the Effective Time, each Company Option that was then outstanding was automatically cancelled and, if such Company Option had a per share exercise price less than the Merger Consideration, the holder thereof became entitled to receive a cash payment, without interest thereon and subject to any applicable tax withholding, equal to the product obtained by multiplying (i) the total number of shares of Company Common Stock underlying such Company Option by (ii) the excess of the Merger Consideration over the exercise price per share such Company Option. ( 8 )(Continued from Footnote 7) Any Company Option that had an exercise price per share that was equal to or exceeded the Merger Consideration was cancelled without any cash payment or other consideration being made in respect thereof. ( 9 )1/12th of the shares subject to the option become vested and exercisable on February 2, 2024 and 1/12 of the shares subject to the option shall vest monthly thereafter.

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